SANGER SEQUENCING AND ITS RECENT ADVANCES - A REVIEW

Abstract

The main aim of the study is to analyse the role of Sanger sequencing and its recent advances .DNA sequencing has revolutionized biomedicine, and progress in the field has been unrelenting since it was invented over 30years ago. As a complete DNA sequence of the human genome is obtained from decades of work by a large number of scientists. Less than a decade later the next-generation' instruments can make it possible for a single lab to produce the same amount of data in a week. The instruments are now increasingly automated, upstream sample processing remains a challenge. An extensive review of literature of Sanger sequencing and its advances by collection of data Pubmed, core, google scholar, Cochran and semantic scholar base medicine Gor-Mesk from the articles dates from 2000 - 2020.From which data is analysed to interpret the results.Inclusion criteriaArticles related to Sanger sequencing , human genome amplification, DNA profiling methods for forensic identification and Advanced DNA sequencing techniques.Exclusion criteria : articles related to other
categories.The recent articles discussed in this review helps in attaining knowledge and awareness about Sanger sequencing and its recent advanced techniques. Advanced DNA sequencing techniques such as metagenomics, transcriptome sequencing, genome sequencing, re-sequencing, chromatin immunoprecipitation and diagnosis of genetic diseases in human genetic history.This review gives a clear view about knowledge and awareness about Sanger sequencing and its advances , advantages and uses in medical field,genetics and forensic medicine.