EFFECT OF UNBALANCED IN OXIDATIVE STRESS AND ANTIOXIDANT STATUS ON RBCS MEMBRANES IN THYROID DISORDERS PATIENTS

Abstract

Many hypothyroid and hyperthyroid patients are anemic because the changes in the concentration of thyroid hormones result in the increased generation of reactive oxygen species (ROS) and oxidative stress leading to changes within the erythrocytes membrane; during this study, the osmotic fragility of the red blood cells and proteins of their membrane from non-treated hypo and hyperthyroid patients diagnosed on the idea of clinical examinations and paraclinical data were compared with a control group. The study included 140 samples collected from patients who suffer from thyroid disorders and 100 samples collected from healthy people as a control group. Ranged in age from patients and healthy individuals (17-79) years. Hormones, antioxidants, and malondialdehyde were measured by ELISA methods; whilethe osmotic fragility and erythrocyte membrane proteins were measured according to the standard method.Significant changes were observed in the level of hormones, TSH increased in hypothyroidism and decreased in hyperthyroidism while T3 and T4 increased in hyperthyroidism and decreased in hypothyroidism. CAT, SOD, and GR were changed significantly that increased in hyperthyroidism and decreased in hypothyroidism. No significant changes were observed in the osmotic fragility in hypothyroidism but showed significant changes in hyperthyroidism in the 0.45-0.9 NaCl; Whereas erythrocyte membrane proteins

decreased significantly in both cases. The present study confirmed increased oxidative stressand impairment of the antioxidant system in hypo and hyperthyroidism and its effect on the erythrocyte membrane by increasing osmotic fragility and decreasing membrane proteins.