EVALUATE THE RELATION BETWEEN LUXS GENE AND THE BIOFILM PRODUCTION BY KLEBSIELLA PNEUMONIAE‏

Abstract

Abstract

The quorum sensing system control by the output, secretion and identification of extracellular signal molecules called auto-inducers. The Gram-negative bacterium Klebsiella pneumoniae consider as a multidrug-resistant pathogen that spread globally. Biofilms provide conditions that shield cells from adverse environmental influences, for this reason this study aimed to detect the relation between one of the QS gene (LUX). One hundred tewnty isolates were collected from patients suffered from pneumonia infection but only 22 isolates were K. pneumonia by the API system. Biofilm production were calculate and RNA were extract and then converted to cDNA to perform real time- PCR done for those samples to detect the gene expression. the results showed that only 17 samples possess the LUX gene and The LUX gene does not contain 5 isolates and those five isolates showed a lower biofilm production level. While 17 isolates indicates a higher level of development of biofilm, additionally, a positive and significant correlation were confirm between biofilm production and LUX gene expression. From the above approach results, we can conclude that, LUX gene effect strongly on the production of biofilm and the rate of LUX gene expression positively correlated with biofilm production.