ANTIOXIDANT ACTIVITIES OF PHALERIA MACROCARPA FRUITS EXTRACTED BY AQUEOUS EXTRACTION METHOD

Abstract

Alcohol and chloroform were common solvents used for extraction, and might cause harmful
environmental effect if not handle the disposal properly. This study was carried out to determine
the antioxidants activity if P.macrocarpa fruit which extracted by aqueous solvent. The
extraction was carried out using Soxhlet extraction method. Crude extracted were then
measured its antioxidants activities and capacities, by using three different assay, consists of
Free Radical Scavenging Activity and its half maximal inhibitory concentration, Ferric Reducing
Power Assay, and Total Phenolic Content. The results obtained from the analysis were 80.114%
scavenging activity, 92.95% of half maximal inhibitory at concentration of 62.5 mg/mL, 5199.02
µmol Fe2+/100 mL for reducing power of ferric, and 369.1 mg GAE/g for total phenolic content.
Since water is polar solvent, it gives significant results of antioxidants activities from extracted
sample. It showed that the presence of phytochemicals from P.macrocarpa extract was
significantly high and the aqueous solvent used does not lowered the antioxidants readings.