STUDY OF THE EXPRESSION OF CATALASE (CAT), ASCORBATE PEROXIDASE (ASCP), GLUTATHIONE REDUCTASE (GR) GENES IN SOYBEAN DROUGHT-TOLERANT AND SENSITIVE CULTIVARS USING REAL-TIME PCR

Abstract

Drought is one of the major constraints that limit agricultural productivity. Some factors, including climate changes and acreage expansion, indicate the need for developing drought-tolerant Genotypes. for the Study of the expression of Catalase (CAT), Ascorbate peroxidase (Ascp), Glutathione Reductase (GR) genes in soybean drought-tolerant and sensitive cultivars using real-time PCR. Seeds from Karbin (drought-sensitive) and Fora (drought-tolerant) lines were planted under specific temperature conditions at two levels of normal irrigation conditions and drought stress treatment, in the research greenhouse of Islamic Azad University of Arak, Iran. Changes in gene expression compared to control samples were recorded using the formula 2^-ΔΔCT. Three technical replications were given for each cDNA sample related to each sampling and used to analyze test data from Excel software. The results showed that the expression threshold of (Glutathione) gene expression was lower than the reference gene in both treatments (stress treatment and normal conditions) than the reference gene expression Still resistant line had more name in stress treatment and normal conditions treatment. The Glutathione gene, like the CAT gene in the resistant this result showed that the expression of GR gene, like the expression of CAT gene in resistant line before stress treatment, was higher than the sensitive line. The theory is also correct that chaperone proteins produced during the plant growth cycle do not disappear to express the Glutathione Reductase gene. The ASCP gene expression cycle indicates that the proteins produced by this gene have a high rate of expression before stress in cells.