STRATEGY FOR DETECTION OF JAPANESE ENCEPHALITIS VIRUS GENOTYPE WITH COMBINATION MAC-ELISA, NS1-ELISA, AND REALTIME RT-PCR
Japanese encephalitis virus (JEV) is a mosquito-borne virus, that causes acute encephalitis syndrome (AES). Suspected viral AES patients in General hospital Bac Giang province, 2004-2017 were selected 310 patients, clinical samples were collected for JE diagnosis by NS1-ELISA and MAC-ELISA as a strategy to select positive samples for genotyping by real-time RT-PCR and sequencing. The results showed that in acute samples, 81/320 (25,31%) JE cases were detected by MAC-ELISA, but only 3,75% (12/320) JE cases by NS1-ELISA. Thus, the sensitivity of NS1-ELISA is far from MAC-ELISA (k=0.4). But it is useful for selecting samples for the genotype of JEV. By Realtime RT-PCR yielding 4/12 CSFs were positive with JEV primer sets I and III, showing JEV genotypes I and III cause human disease in Bac Giang province from 2004 to 2017. The sequence of whole E gene genotype III of an isolate coded 04VN76 (isolated from CSFs coded 04 CSF98) was decoded and submitted on Genbank.